Mitsuaami was originally several people at a hair salon, turned into a monster by Malachite. She attempted to track Sailor Moon through her hair, but one of Serena's hairs got on Mina's hair, leading Mitsuaami to believe that she was Sailor Moon. When the real Sailor Moon arrived, she fought Mitsuaami, but Prince Darien, how under the control of the Negaverse, arrived to help Mitsuaami fight Sailor Moon. In the end, Mitsuuami was attacked by the other Sailor Scouts before being healed by Sailor Moon using Moon Healing Activation, turning her back into all the people at the hair salon.
Her name is a pun on "Mitsuami" (三つ編み) which refers to the Japanese word for hair "braids" or "plait".